Department of Histology, Cytology and Embryology

KHARKIV NATIONAL MEDICAL UNIVERSITY

MAIN PAGE

About Department

Topics of the practical classes

General morphology of the cell. Liver cells (hepatocytes). Haematoxylin & eosin, X400. 1. – cytoplasm 2. – nucleus 3. – nucleolus

Golgi complex in the neurons of the dorsal root ganglion. Osmium tetroxide, X400 1. nucleus 2. nucleolus 3. Golgi complex

Glycogen inclusions in the liver cells. Best’s carmine staining, X400. 1. clumps of glycogen

Lipid inclusions in the liver cells. Safranin staining after fixation with osmium tetroxide, X400. 1. cells boundaries 2. nuclei 3. lipid inclusions

Pigment inclusions in the melanocytes. not stained, X400. 1. Processes of melanocytes 2. Nuclei

Mitosis. Iron haematoxylin staining, X100. 1. The beginning of prophase 2. Metaphase 3. Late anaphase 4. Telophase 5. Interphase

Ovum. Х400 1. Nucleus 2. Yolk inclusions 3. Zona pellucida 4. Corona radiata

Guinea pig’s spermatozoa. Iron hematoxylin staining. X400. 1. Head with the nucleus inside 2. Acrosome 3. Neck 4. Tail

Frog’s blastula. Haematoxylin & picrofucsin staining, X100. 1. Vegetative pole 2. Аnimal pole 3. Blastocoele

Primitive streak in the chicken embryo. Hematoxylin staining, X40 1. Ectoderm 2. Endoderm 3. Primitive groove 4. Primitive streak 5. Mesoderm

Embryonic axial organs. Iron hematoxylin staining., X40. 1. Ectoderm 2. Endoderm 3. Neural tube 4. Notochord 5. Somites 6. Nephrotome 7. Somatopleurea(parietal layer of splanchnotome) 8. Splanchnopleura (visceral layer of splanchnotome) 9. Mesenchyme 10. Coelom 11. Blood vessels

Chicken embryo at the stage of formation of trunk and amniotic folds (transverse section, coloration with iron hematoxylin), X40 1.Trunk fold 2. The intestinal cecum 3. Chorda 4. Nervous tube 5. Skin ectoderm 6.Dermatomes 7. Formation of renal tubules at the site of nephrotomes 8. Overall 9. The parietal sheet of the splanchnotome 10. Visceral sheet of splanchnotome 11. Blood vessels 12. Amniotic fold

Simple cuboidal epithelium. Haematoxylin & eosin staining, X400 * - epithelial cells 1. Apical pole 2. Basal pole 3. Connective tissue

Simple columnar epithelium. haematoxylin and eosin staining, X400 1. Epithelial cells 2. Basal pole 3. Apical pole 4. Blood vessels 5. Basal lamina

Simple squamous epithelium (mesothelium). Silver nitrate impregnation and haematoxylin staining, X400. 1. Cells boundaries 2. Nuclei

Pseudostratified columnar ciliated epithelium. iron haematoxylin, X400. 1. Nuclei 2. Microvilli 3. Basal bodies of microvilli 4. Connective tissue

Transitional epithelium of the urinary bladder. Haematoxylin and eosin staining, X100. 1. Basal layer 2. Connective tissue 3. Intermediate cell layer 4. Surface cell layer

Transitional epithelium of the urinary bladder. Haematoxylin and eosin staining, X400. 1. Basal layer 2. Connective tissue 3. Intermediate cell layer 4. Surface cell layer

Simple tubular glands of the cat’s uterus. Haematoxylin and eosin staining, Х100. 1. Glands 2. Cuboidal epithelium 3. Сonnective tissue

Unicellular endoepithelial glands ( Leydig cells) and simple alveolar extraepithelial glands. Slide is made from Axolotl skin, Х100. Hematoxylin and eosin staining. 1. Leydig cells 2. Сonnective tissue 3. Simple alveolar unbranched extraepithelial glands 4. Excretory duct 5. Pigment cells

Chicken embryo mesenchyme. iron haematoxylin, Х400. * – Mesenchyme 1. Cells nuclei 2. Cells processes

Human blood smear. Blood smear is stained by Romanowsky-Giemsa’s method, X400. 1. Erythrocytes 2. Platelets 3. Segmented neutrophils 4. Band neutrophils 5. Lymphocytes 8. Monocytes

Human blood smear. Blood smear is stained by Romanowsky-Giemsa’s method, X400. 1. Erythrocytes 3. Segmented neutrophils 4. Band neutrophils 5. Lymphocytes

Human blood smear. Blood smear is stained by Romanowsky-Giemsa’s method, X400. 3. Segmented neutrophils 8. Monocytes

Human blood smear. Blood smear is stained by Romanowsky-Giemsa’s method, X400. 6. Eosinophil

Human blood smear. Blood smear is stained by Romanowsky-Giemsa’s method, X1000. Lymphocyte.

Human blood smear. Blood smear is stained by Romanowsky-Giemsa’s method, X1000. Monocyte.

Human blood smear. Blood smear is stained by Romanowsky-Giemsa’s method, X1000. Segmented neutrophils.

Human blood smear. Blood smear is stained by Romanowsky-Giemsa’s method, X1000. Eosinophil.

Loose connective tissue of the rat. Iron haematoxylin staining, Х100. *- ground substance 1. Collagen fibers 2. Elastic fibers 3. Fibroblasts 4. Macrophages

Loose connective tissue of the rat. Iron haematoxylin staining, Х400. 5. Tissue lymphocytes 6. Plasma cells.

Loose connective tissue of the rat. Iron haematoxylin staining, Х400. *- ground substance 1. Collagen fibers 3. Fibroblasts

Loose connective tissue of the rat. Iron haematoxylin staining, Х400. *- ground substance 1. Collagen fibers 2. Elastic fibers 5. Tissue lymphocytes

Tendon (longitudinal section), Х100 Hematoxylin and eosin staining. 1.Primary bundles of collagen fibers 2.Tendinocyte nuclei 3.Secondary bundles of collagen fibers (tendon fascicles) 4.Endotendineum

Ligament(longitudinal section) Slide is stained with picric acid, acid fuchsin and hematoxylin, Х100 1.Elastic fibers 2.Collagen fibers 3.Fibroblasts

Ligament(longitudinal section) Slide is stained with picric acid, acid fuchsin and hematoxylin, Х40 1.Elastic fibers 2.Collagen fibers 3.Fibroblasts

Reticular tissue of lymph node Hematoxylin and eosin staining, Х100 *- Reticular cells 1.Cytoplasm and processes 2.Cells nuclei 3.Lymphocytes

Adipose tissue To identify the fat inclusions slide is stained with sudan and Hematoxylin, Х100 1.Adipocytes 2.Lipid drop

Hyaline cartilage Hematoxylin and eosin staining, Х100 1.Perichondrium 2.Extracellular matrix 3.Zone of young chondrocytes 4.Mature chondrocytes (isogenous groops)

Elastic cartilage Slide is stained with orcein and hematoxylin, Х100 1.Perichondrium 2.Ground substance 3.Elastic fibers 4.Isogenic cells groops

Fibrous cartilage Hematoxylin and eosin staining, Х100 1.Bundles of collagen fibers 2.Ground substance 3.Chondrocytes

Сompact bone (transversus section) Slide is stained by Schmorl’s method with Thionine and picric acid ), Х40 1.Periosteum 2.Outer circumferential lamellae 5.Oteonal canals 6.Osteons lamellae

Сompact bone (transversus section) Slide is stained by Schmorl’s method with Thionine and picric acid ), Х100 2.Outer circumferential lamellae 5.Oteonal canals 6.Osteons lamellae

Сompact bone (transversus section) Slide is stained by Schmorl’s method with Thionine and picric acid ), Х400 4.Inner circumferential lamellae

Сompact bone (transversus section) Slide is stained by Schmorl’s method with Thionine and picric acid ), Х40 1.Periosteum 5.Oteonal canals 6.Osteons lamellae 7.Interstitial lamellae